100-μm depth chamber slide Search Results


90
Klarmann Rulings eyepiece microruler
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ibidi GmbH microfluidic parallel platelet flow chamber
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
Microfluidic Parallel Platelet Flow Chamber, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fisher Scientific glass slides
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
Glass Slides, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher heart organoid medium
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
Heart Organoid Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StatLab Medical Products Inc glass slides
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
Glass Slides, supplied by StatLab Medical Products Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Neuralynx inc polyimide tubing
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
Polyimide Tubing, supplied by Neuralynx inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences glass slides corning
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
Glass Slides Corning, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Selleck Chemicals 5 fu
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
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VitroCom Inc microchamber slide htr1099
( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a <t>microfluidic</t> flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).
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Image Search Results


( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a microfluidic flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).

Journal: Science Advances

Article Title: Reduced platelet forces underlie impaired hemostasis in mouse models of MYH9 -related disease

doi: 10.1126/sciadv.abn2627

Figure Lengend Snippet: ( A ) Lysis onset time (LOT) and lysis time (LT) assessed with rotational thromboelastometry (ROTEM) without treatment (−), stimulation with rtPA, and after the addition of TXA to contrast rtPA-induced lysis stimulation. Overlapping of the modified ROTEM analysis curves (green, no treatment; red, rtPA stimulation; blue, rtPA and TXA stimulation). ( B ) Representative fluorescence images of human platelet thrombi (CD61; green) and fibrin (magenta) at 20 min after treatment in a microfluidic flow chamber at a shear of 100 s −1 (scale bars, 50 μm). ( C ) Time course of changes in stability of fibrin/-ogen (normalized fluorescence of fibrin/-ogen AF647; mean ± SD) on sites of platelet thrombi after the addition of TXA (100 μM) compared to nontreated control. ( D ) Whole blood from healthy individuals and an MYH9 p.D1424N patient was examined (untreated, with rtPA, with TXA, and with TXA + rtPA) using colloidal probe spectroscopy. Each data point shows the median Young’s modulus of one healthy individual or MYH9 p.D1424N aggregate, and bar plots show means ± SD of Young’s modulus. Statistics: Mann-Whitney U test (ns, P ≥ 0.05; *0.05 > P ≥ 0.01; and *** P < 0.001).

Article Snippet: Ex vivo thrombus formation assays were performed at a wall shear rate of 1000 s −1 on collagen-passivated surfaces [Horm collagen type I (200 μg/ml) from horse tendon; Nycomed] in a microfluidic parallel platelet flow chamber (on μ-Slide VI 0.1 with the following physical dimensions: width, 1 mm; height, 100 μm; and length, 17 mm; ibidi GmbH, Germany).

Techniques: Lysis, Modification, Fluorescence, Shear, Control, Spectroscopy, MANN-WHITNEY